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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might lead to tumorigenesis. This protein was observed to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant role in advertising mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two further splice variants had been documented but thorough nucleotide sequence facts isn't but out there.
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Blocking peptides are peptides that bind precisely to your focus on antibody and block antibody binding. These peptide typically incorporates the epitope acknowledged because of the antibody.
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This information outlines additional doable optimization of cellular and immunoassay parameters to be sure the best possible success are...
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This guide outlines more doable optimization of cellular and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked click here antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to the highly conserved cyclin spouse and children, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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